HPLC Column, IEX WCX, 5um, 4.6mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent IEX WCX is a weak cation exchange (WCX) HPLC stationary phase designed for the retention and separation of positively charged compounds. Manufactured using high-purity silica and carboxylic acid functional groups, the stationary phase provides a negatively charged surface within its functional pH range of approximately 2.5 to 7, enabling reliable cation exchange selectivity for basic compounds and other positively charged analytes. This popular chromatography phase has been widely used in HPLC laboratories for many years and serves as an excellent alternative for many USP L9 methods.
67591-25P is a 250 mm x 4.6 mm ID analytical HPLC column packed with 5 µm WCX particles with a 100 Å pore size. The extended 250 mm column length provides maximum resolving power, increased theoretical plate counts, and enhanced separation of closely related compounds in demanding ion exchange applications. The 4.6 mm ID format is the industry standard for conventional HPLC systems and typically operates at approximately 1.0–2.0 mL/min, making it ideal for impurity profiling, complex sample analysis, and methods requiring the highest levels of chromatographic resolution.
This weak cation exchange column is particularly useful for the analysis and purification of basic pharmaceuticals, amines, peptides, proteins, and other positively charged compounds. The combination of selective WCX functionality, high-purity silica, efficient 5 µm particles, and extended column length delivers exceptional chromatographic performance for challenging cation exchange separations where maximum resolution and reproducibility are required.
Key Benefits
Weak cation exchange (WCX) stationary phase
Carboxylic acid functional groups
Suitable for positively charged analytes
Alternative for many USP L9 methods
High-purity silica support
5 µm particles for efficient separations
Maximum chromatographic resolution from 250 mm column length
Increased theoretical plate counts
Excellent reproducibility and ion exchange selectivity
Typical Applications
Ion exchange chromatography
Cation separations
Basic compound analysis
Pharmaceutical analysis
Amine separations
Peptide analysis
Protein analysis
USP L9 methods
Impurity profiling
Method validation
Purification of positively charged compounds
Complex sample separations
Specifications
Product Type: HPLC Column
Stationary Phase: IEX WCX
USP Classification: L9
Chromatography Mode: Weak Cation Exchange
Functional Group: Carboxylic Acid
Particle Size: 5 µm
Pore Size: 100 Å
Column Dimensions: 4.6 mm ID x 250 mm
Length: 250 mm
Recommended Flow Rate: 1.0–2.0 mL/min
Brand: Cogent
Important Notes
Designed for weak cation exchange chromatography
Retains positively charged analytes
Functional charged range approximately pH 2.5–7
Suitable for many USP L9 applications
High-purity silica provides reliable chromatographic performance
Longer column length provides maximum chromatographic resolution
Ideal for impurity profiling and complex separations
Compatible with conventional HPLC systems